rabbit anti usp24  (Bethyl)

Journal: Cell chemical biology

Article Title: A Rapid and Precise Mutation-Activated Fluorescence Reporter for Analyzing Acute Mutagenesis Frequency

doi: 10.1016/j.chembiol.2018.05.010

Figure Lengend Snippet: (A) Representative red-green flow diagrams of HCT116 cells carrying CherryOFF-GFP and USP24 KD or nonsilencing shRNA control, exposed to 10 or 40 J/m2 UV radiation, or untreated. UV irradiation increased mutagenesis in both cell lines, and higher mutagenesis was seen in USP24 KD.

Article Snippet: USP24 knockdown efficiency was quantified via western blot using a rabbit-anti-USP24 antibody (Origene TA5240) compared to tubulin control (Santa Cruz H-235)

Techniques: shRNA, Control, Irradiation, Mutagenesis

Journal: Cell chemical biology

Article Title: A Rapid and Precise Mutation-Activated Fluorescence Reporter for Analyzing Acute Mutagenesis Frequency

doi: 10.1016/j.chembiol.2018.05.010

Figure Lengend Snippet: (A) USP24 KD HCT116 cells treated with 10 J/m2 UV, and ATE1-KO MEF cells treated with 40 J/m2, were sorted and cultured. Representative picture shown with arrow indicating one cell exhibiting only GFP signal in the sorted HCT116 cells. Scale bar represents 100 µm. As shown in the table (right), we found less than 1% green-only cells in HCT cells that were sorted for red fluorescence, which may be due to sticky cell doublets during FACS. In the less self-adhesive MEF cells, a much lower percentage of green-only cells was found in the CherryOFF-GFP carrying cells that were sorted for red signal. Mean cell numbers are shown (±SEM) from three independent experiments.

Article Snippet: USP24 knockdown efficiency was quantified via western blot using a rabbit-anti-USP24 antibody (Origene TA5240) compared to tubulin control (Santa Cruz H-235)

Techniques: Cell Culture, Fluorescence, Adhesive

Journal: Cell chemical biology

Article Title: A Rapid and Precise Mutation-Activated Fluorescence Reporter for Analyzing Acute Mutagenesis Frequency

doi: 10.1016/j.chembiol.2018.05.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: USP24 knockdown efficiency was quantified via western blot using a rabbit-anti-USP24 antibody (Origene TA5240) compared to tubulin control (Santa Cruz H-235)

Techniques: Virus, shRNA, Recombinant, Isolation, SYBR Green Assay, Software

Journal: Cell chemical biology

Article Title: A Rapid and Precise Mutation-Activated Fluorescence Reporter for Analyzing Acute Mutagenesis Frequency

doi: 10.1016/j.chembiol.2018.05.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-USP24 , Origene , Cat#TA315240; RRID: AB_2722752.

Techniques: Virus, shRNA, Recombinant, Isolation, SYBR Green Assay, Software